Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-15 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduJK BMC Components
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K562004_sequence (Version 1) |
Description
This is a composite part comprising a constituive promoter (identical to that from the basic part BBa_K562000), which is the tatABCD promoter from E. coli K-12, driving production of the PduJ and PduK proteins from Salmonella enterica serovar Typhimurium LT2. These are components of the Salmonella bacterial microcompartment (BMC). The PduK gene product carries a C-terminal 6-His affinity/epitope tag. Production of the PduK protein has been verified by Western immunoblotting (anti-penta-His) and production of both PduJ and PduK have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-JK in the Sargent Laboratory, Dundee, UK.Notes
The fragment carries an engineered BamHI site at its extreme 3' end, but note that this is because a generic primer design system was used. Be aware that the pduK gene carries 2 native BamHI sites.Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562000 synthetic pduJ gene pduK gene Hexa-Histidine Tag | 1,103 107,112 119,394 419,877 878,895 | feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/cds CDS feature/cds CDS feature/misc sequence_feature |