Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-15 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduABTU BMC Components
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K562007_sequence (Version 1) |
Description
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the PduA and PduB proteins from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562003) AND proteins PduT and PduU. These are all components of the Salmonella bacterial microcompartment (BMC). The PduB and PduU gene product carry C-terminal 6-His affinity/epitope tags. Production of the PduB and PduU proteins has been verified by Western immunoblotting (anti-penta-His) and production of PduA, PduB, PduT and PduU have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-ABTU in the Sargent Laboratory, Dundee, UK.Notes
Native PstI sites in pduB and pduU were removed by site-directed mutagenesis. The fragment also carries an engineered BamHI site at its extreme 3' end.Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562003 synthetic RBS pduA gene pduB gene Hexa-Histidine Tag synthetic RBS pduT gene pduU gene Hexa-Histidine Tag | 1,1236 107,112 119,403 400,1209 1210,1227 1240,1245 1252,1806 1806,2153 2154,2171 | feature/BioBrick engineered_region ribosome_entry_site feature/rbs CDS feature/cds CDS feature/cds feature/misc sequence_feature ribosome_entry_site feature/rbs CDS feature/cds CDS feature/cds feature/misc sequence_feature |