Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-15 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduABTUNJK BMC Components
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K562009_sequence (Version 1) |
Description
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the PduA, PduB, PduT, PduU and PduN proteins from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562008) AND proteins PduJ and PduK. These are all components of the Salmonella bacterial microcompartment (BMC). The PduB, PduU, PduN and PduK gene products all carry C-terminal 6-His affinity/epitope tags. Production of the PduB, PduU, PduN and PduK proteins has been verified by Western immunoblotting (anti-penta-His) and production of PduA, PduB, PduT, PduU, PduN, PduJ and PduK has been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-ABTUNJK in the Sargent Laboratory, Dundee, UK.Notes
Native PstI sites have been removed from pduB and pduU. There is an engineered BamHI site at the extreme 3' end, but please note that pduK contains two native BamHI sites that have not been removed.Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562008 synthetic RBS pduA gene pduB gene Hexa-Histidine Tag synthetic RBS pduT gene pduU gene Hexa-Histidine Tag synthetic RBS pduN gene Hexa-Histidine Tag synthetic RBS pduJ gene pduK gene Hexa-Histidine Tag | 1,2495 107,112 119,403 400,1209 1210,1227 1240,1245 1252,1806 1806,2153 2154,2171 2184,2189 2196,2468 2469,2486 2499,2504 2511,2786 2811,3269 3270,3287 | feature/BioBrick engineered_region feature/rbs ribosome_entry_site feature/cds CDS feature/cds CDS feature/misc sequence_feature feature/rbs ribosome_entry_site feature/cds CDS feature/cds CDS sequence_feature feature/misc ribosome_entry_site feature/rbs feature/cds CDS feature/misc sequence_feature feature/rbs ribosome_entry_site CDS feature/cds feature/cds CDS feature/misc sequence_feature |