Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduD20-mCherry
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K562010_sequence (Version 1) |
Description
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 20 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562001), which is itself fused in-frame to mCherry. The mCherry gene product also carries a C-terminal HA epitope tag. Production of PduD20-mCherry has been verified by Western immunoblotting (anti-mCherry). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D20-mCh in the Sargent Laboratory, Dundee, UK.Notes
The single natural pstI site within the mCherry gene has been removed.Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and an unknown cloning vector encoding mCherry.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562001 synthetic RBS PduD20 Tag mCherry HA epitope tag | 1,184 107,112 119,178 185,889 890,916 | feature/BioBrick engineered_region ribosome_entry_site feature/rbs feature/cds CDS feature/cds CDS feature/misc sequence_feature |