Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-16 11:00:00
Date modified: 2015-05-08 01:12:41
Salty_PduD40-mCherry
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K562011_sequence (Version 1) |
Description
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to mCherry. The mCherry gene product also carries a C-terminal HA epitope tag. Production of PduD40-mCherry has been verified by Western immunoblotting (anti-mCherry). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-mCh in the Sargent Laboratory, Dundee, UK.Notes
The natural pstI site within mCherry has been removed.Source
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and an unknown cloning vector.| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562002 synthetic RBS PduD40 Tag mCherry HA epitope tag | 1,244 107,112 119,238 245,949 950,976 | engineered_region feature/BioBrick feature/rbs ribosome_entry_site tag feature/tag feature/cds CDS feature/tag tag |