PduD20-GFP

BBa_K562012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K562012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Frank Sargent

Date created: 2011-09-16 11:00:00

Date modified: 2015-05-08 01:12:41

Salty_PduD20-GFP

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K562012_sequence (Version 1)

Description

This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 20 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562001), which is itself fused in-frame to GFP. The GFP gene product also carries a C-terminal HA epitope tag. Production of PduD20-GFP has been verified by Western immunoblotting (anti-GFP). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D20-GFP in the Sargent Laboratory, Dundee, UK.

Notes

None

Source

Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and an unknown cloning vector encoding GFP.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K562001	1,184	feature/BioBrick, engineered_region
synthetic RBS	107,112	feature/rbs, ribosome_entry_site
PduD20 Tag	119,178	CDS, feature/cds
GFP	185,895	feature/cds, CDS
HA epitope tag	896,922	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K562012/1

Member of these Collections

• iGEM Parts Registry