PduD40-Xyl

BBa_K562016 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K562016

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Frank Sargent

Date created: 2011-09-17 11:00:00

Date modified: 2015-05-08 01:12:41

Salty_PduD40-XylA

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K562016_sequence (Version 1)

Description

This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to D-xylose isomerase (XylA) from Escherichia coli K-12. The XylA gene product also carries a C-terminal HA epitope tag. Production of PduD20-XylA has been verified by Western immunoblotting (anti-HA). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-XylA in the Sargent Laboratory, Dundee, UK.

Notes

None

Source

Derived from Salmonella enterica serovar Typhimurium LT2 and Escherichia coli K-12 MG1655 genomic sequences.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K562002	1,244	feature/BioBrick, engineered_region
synthetic RBS	107,112	feature/rbs, ribosome_entry_site
PduD40 Tag	119,238	tag, feature/tag
XylA	245,1586	CDS, feature/cds
HA epitope tag	1587,1613	tag, feature/tag

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K562016/1

Member of these Collections

• iGEM Parts Registry