Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frank Sargent
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:42
Salty_PduD40-GFPssrA
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K562019_sequence (Version 1) |
Description
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the initial 40 residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562002), which is itself fused in-frame to GFP. The GFP gene product also carries a C-terminal ssrA degradation tag. Production of PduD40-GFP-ssrA has been verified by Western immunoblotting (anti-GFP) when co-expressed with BBa_K562009 encosing a bacterial microcompartment (BMC). The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D40-GFPssrA in the Sargent Laboratory, Dundee, UK.Notes
NoneSource
Derived from Salmonella enterica serovar Typhimurium LT2 genomic sequence and a plasmid described in this paper:DeLisa MP, Samuelson P, Palmer T, Georgiou G. Genetic analysis of the twin arginine translocator secretion pathway in bacteria. J Biol Chem. 2002 Aug 16;277(33):29825-31.
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K562002 synthetic RBS PduD40 Tag GFP ssrA degradation tag | 1,244 107,112 119,238 245,955 962,994 | feature/BioBrick engineered_region ribosome_entry_site feature/rbs feature/tag tag feature/cds CDS feature/tag tag |