BBa_K563002

BBa_K563002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K563002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Boxuan Zeng

Date created: 2011-10-04 11:00:00

Date modified: 2015-05-08 01:12:42

promoter of ILV2

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K563002_sequence (Version 1)

Description

Promoter of ILV2, under control of transcription factor Gcn4. In our project, when When TORC1 is inhibited by FAP, dephosphorylated Gcn4 would bind with pHIS4 and activate downstream genes. We put the target gene of mutant Tor2 under this promoter, and then its expression could be controlled by the amount of FAP and the activity of TORC1. Mutant Tor2 would be much resistant to FAP and a feedback loop is formed between FAP, TORC1, Gcn4 and mTor.

Notes

Design NcoI and XhoI at two ends of the promoter

Source

Amplified from the genome of S.cerevisiae by PCR

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
promoter of ILV2	1,509	promoter, feature/promoter

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K563002/1

Member of these Collections

• iGEM Parts Registry