Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Guo Jing
Date created: 2011-10-04 11:00:00
Date modified: 2015-05-08 01:12:42
pEASY+22Tor2(with L->P mutation)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K563034_sequence (Version 1) |
Description
There are totally four mutation sites as we designed in the complete Tor2 sequence. This BioBrick contains the second part of Tor2 with mutation that from amino acid L to amino acid P.Notes
We will use overlap PCR to conduct this mutation. After we get this mutant part of Tor2, we can keep it for further connection.Source
pEASY vector is bought from Transgene Company, and we get mutant-22Tor2by mutating the 22Tor2 from genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| 22Tor2(LP mutation) Ampicillin Resistance ORF Kanamycin Resistance ORF LP mutation | 294,3002 4838,5698 4026,4820 2173,2175 | feature/cds CDS CDS feature/cds feature/cds CDS feature/dna sequence_feature |