Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Guo Jing
Date created: 2011-10-04 11:00:00
Date modified: 2015-05-08 01:12:42
pEASY+33Tor2(with V->A mutation)
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K563036_sequence (Version 1) |
Description
There are totally four mutation sites as we designed in the complete Tor2 sequence. This BioBrick contains the third part of Tor2 with mutation that from amino acid V to amino acid A.Notes
We will conduct this mutation by designing primers with mutant site. After we get this mutant part of Tor2, we can keep it for further connection.Source
pEASY vector is bought from Transgene Company, and we get mutant-33Tor2 by mutating the 33Tor2 from genomic sequence.| Sequence Annotation | Location | Component / Role(s) |
| Ampicillin resistance ORF 33Tor2 (VA mutation) Kanamycin resistance ORF VA mutation site | 4628,5488 294,2792 3816,4610 1672,1674 | CDS feature/cds feature/cds CDS CDS feature/cds feature/dna sequence_feature |