Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Guo Jing
Date created: 2011-10-04 11:00:00
Date modified: 2015-05-08 01:12:42
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K563053_sequence (Version 1) |
Description
pYES2 is a 5.9 kb vector for inducible expression of recombinant proteins in S.cerevisivae. Feature of the vector allow easy cloning of your gene of interest and selection of transformants by uracil prototrophy. The vector contains the follow elements:Notes
We use this vector for expressing mutant Tor2. In order to operate easily, we separate Tor2 into three parts. So we reconstruct the pYE by adding specific restriction sites for linking.Source
we bought it from invitrogen| Sequence Annotation | Location | Component / Role(s) |
| GAL1 CYC1 transcription terminator Amplicillin URA3 gene pUC origin f1 origin | 1,451 608,856 1856,2716 2734,3841 1038,1711 5384,5839 | promoter feature/promoter stop_codon feature/stop feature/cds CDS feature/cds CDS feature/dna sequence_feature sequence_feature feature/dna |