Pbad-lacZ

BBa_K564009 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K564009

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Damian Rafal Plichta

Date created: 2011-09-19 11:00:00

Date modified: 2015-05-08 01:12:42

Arabinose promoter fused with lacZ

Details

• Types: DnaRegion
• Roles: engineered_region; Measurement
• Sequences: BBa_K564009_sequence (Version 1)

Description

Para (PBAD) is an E. coli promoter that is induced by the presence of arabinose in the medium. In this part it is fused with lacZ which encodes β-galactosidase (an intracellular enzyme that cleaves the disaccharide lactose into glucose and galactose). This fusion allows us to check if Para works correctly using β-galactoassays.

Notes

No design consideration

Source

Escherichia coli w3110 genomic DNA

Components

Access	Instance	Definition
public	BBa_I0500	pBad/araC
public	BBa_I732005	LacZ
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500	1,1210	pBad/araC
BBa_I732005	1217,4291	LacZ
BBa_B0010	4300,4379	BBa_B0010
BBa_B0012	4388,4428	BBa_B0012

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K564009/1

Member of these Collections

• iGEM Parts Registry