BBa_K566018

BBa_K566018 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K566018
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Rodriguez
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:43

E. coli MXRed



Types
DnaRegion

Roles
chromosome

Cell

Description

Derived from Dr. Jeff Tabor JT2 E. coli strain, this part is a built-in red-light induction system in E. coli made through chromosome insertion. Avoiding the need of any extra-chromosomal DNA when light-inducing gene expression offering several advantages to the researcher.

It is proposed as a photo-chassis that could make useful tools in this field of light induction.

Integration was performed for the genes pcyA, ho1, Cph8 and a Mnt working as follows:
Genes ho1 and pcyA are responsible for the chromophore synthesis. Cph8 codes for the chimaeric red-light receptor. These three genes are constitutively expressed. Mnt repressor is expressed from pOmpC promoter, which stops being induced in presence of red-light. It is therefore used as a NOT-gate to regulate expression from pMnt.

Notes

Integration protocol of Dr. Kuhlmann

Source

E. coli JT2 strain

Sequence Annotation Location Component / Role(s)
mnt
T
LVA
BBa_C0072
barcode
1,249
95,97
250,282
1,288
289,313
feature/cds CDS
point_mutation feature/s_mutation
tag feature/tag
feature/BioBrick engineered_region
feature/BioBrick engineered_region
igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K566018/1