Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Rodriguez
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:43
Cph8 gene for red-photoreceptor (constitutive expression)
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K566026_sequence (Version 1) |
Description
Cassette for constitutive expression of Cph8, originally constructed by Dr. Jeff Tabor.This gene codifies for the red light-sensing protein Cph8, which is expressed in the phosphorylated ground state. It is switched to the unphosphorylated state by 650-nm light and back to the phosphorylated state by 705-nm light. When phosphorylated, Cph8 passes a phosphoryl group to OmpR, which then binds to and activates transcription from PompC. Because it is inactivated by red light, Cph8 can be considered a logical (NOT red) sensor. A genetic inverter or logical NOT gate is used to invert the response of the (NOT red) sensor to that of a red light sensor
Notes
For the project that this part was made, it was needed to use other Assambly Standard.Source
CDS of Cph8 was extracted by PCR, promoter and terminator were Classic BioBricks.| Access | Instance | Definition |
| public public public | BBa_K081005 BBa_K566025 BBa_B0015 | BBa_K081005 Cph8 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K081005 BBa_K566025 BBa_B0015 | 1,58 65,2299 2308,2436 | BBa_K081005 Cph8 BBa_B0015 |