pRM

BBa_K566760 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K566760
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Daniel Rodriguez
Date created: 2011-09-09 11:00:00
Date modified: 2015-05-08 01:12:43

pRM from Lambda Phage



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K566760_sequence (Version 1)

Description

This part corresponds to the original pRM promoter from Lambda Phage. It contains the OR1, OR2 and OR3 site, therefore, it can be activated and repressed with cI protein from the same phage. This dual regulation is due to cI activates transcription from this promoter when it is on low concentrations, but when cI concentration is higher it represses transcription.

Notes

It did not need any design considerations but including the corresponding BioBrick prefix and sufix into the primers for the PCR.

Source

This part was extracted directly from Lambda Phage genomic DNA using PCR.

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K566760/1