Types | DnaRegion
|
Roles | Regulatory
promoter
|
Sequences | BBa_K566760_sequence (Version 1)
|
Description
This part corresponds to the original pRM promoter from Lambda Phage. It contains the OR1, OR2 and OR3 site, therefore, it can be activated and repressed with cI protein from the same phage. This dual regulation is due to cI activates transcription from this promoter when it is on low concentrations, but when cI concentration is higher it represses transcription.
Notes
It did not need any design considerations but including the corresponding BioBrick prefix and sufix into the primers for the PCR.
Source
This part was extracted directly from Lambda Phage genomic DNA using PCR.