Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bin Zhao
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:43
PT7-TDRS
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K567011_sequence (Version 1) |
Description
aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator. This biobrick is constructed by deleting the anticodon recognition domain of AspRS from E.coli. This modified AspRS can charge Asp to tRNA(Asp)-TAG (BBa_K567013) and tRNA(Asp)-AGG (BBa_K567012).Notes
Use Dicovery Studio to analyze the enzyme and find the tRNA anticodon recognition domain of AspS.Source
aspartyl aminoacyl tRNA synthetase derived from AspS in E.coli| Sequence Annotation | Location | Component / Role(s) |
| lac operator T7 promoter rbs AspRS(truncated) T7 TE | 21,44 2,18 75,81 89,1549 1719,1766 | operator feature/operator promoter feature/promoter feature/rbs ribosome_entry_site CDS feature/cds feature/stem_loop stem_loop |