BBa_K567011

BBa_K567011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K567011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bin Zhao

Date created: 2011-09-28 11:00:00

Date modified: 2015-05-08 01:12:43

PT7-TDRS

Details

• Types: DnaRegion
• Roles: engineered_region; Translational_Unit
• Sequences: BBa_K567011_sequence (Version 1)

Description

aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator. This biobrick is constructed by deleting the anticodon recognition domain of AspRS from E.coli. This modified AspRS can charge Asp to tRNA(Asp)-TAG (BBa_K567013) and tRNA(Asp)-AGG (BBa_K567012).

Notes

Use Dicovery Studio to analyze the enzyme and find the tRNA anticodon recognition domain of AspS.

Source

aspartyl aminoacyl tRNA synthetase derived from AspS in E.coli

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
lac operator	21,44	operator, feature/operator
T7 promoter	2,18	promoter, feature/promoter
rbs	75,81	feature/rbs, ribosome_entry_site
AspRS(truncated)	89,1549	CDS, feature/cds
T7 TE	1719,1766	feature/stem_loop, stem_loop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K567011/1

Member of these Collections

• //cds/enzyme
• iGEM Parts Registry