Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bin Zhao
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:43
tRNA(Asp)-AGG
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K567012_sequence (Version 1) |
Description
tRNA(Asp) with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of lpp promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated.Notes
the correct processing to produce mature tRNA should be ensured.Source
tRNA(Asp) from AspV in E.coli| Sequence Annotation | Location | Component / Role(s) |
| -10 tRNA-Asp GTC->CCT -35 PaspV TE polya | 104,111 138,214 172,174 80,88 80,119 245,267 268,274 | promoter feature/promoter CDS feature/cds sequence_alteration feature/mutation promoter feature/promoter feature/promoter promoter stem_loop feature/stem_loop polyA_site feature/polya |