Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bin Zhao
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:43
tRNA(Asp)-TAG
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K567013_sequence (Version 1) |
Description
tRNA(Asp) with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of lpp promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated.Notes
the correct processing of tRNA should be ensuredSource
the tRNA(Asp) from AspV in E.coli| Sequence Annotation | Location | Component / Role(s) |
| -35 PaspV -10 tRNA-Asp GTC->CTA TE polya | 80,88 80,119 104,111 138,214 172,174 245,267 268,274 | promoter feature/promoter feature/promoter promoter feature/promoter promoter feature/cds CDS sequence_alteration feature/mutation stem_loop feature/stem_loop polyA_site feature/polya |