BBa_K567014

BBa_K567014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K567014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: You Wang

Date created: 2011-09-28 11:00:00

Date modified: 2015-05-08 01:12:43

PT7-metGM

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K567014_sequence (Version 1)

Description

T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator. We have cloned metG from E.coli and have used error-prone PCR to amplify the metG. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and metY-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LB Kana plate.

Notes

Overlapping PCR is used

Source

metG from E.coli. Kana gene derived from pET28a

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T7 promoter	2,18	promoter, feature/promoter
lac operator	21,44	operator, feature/operator
rbs	75,81	feature/rbs, ribosome_entry_site
MetRS(mutated)	89,2122	feature/cds, CDS
T7 TE	2209,2256	feature/stem_loop, stem_loop

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K567014/1

Member of these Collections

• //cds/enzyme
• iGEM Parts Registry