Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: You Wang
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:43
PT7-metGN
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K567015_sequence (Version 1) |
Description
T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator. We have cloned metG from E.coli and the tRNA recognition domain of metG is truncated. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and metY-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LBKana plate.Notes
overlapping PCR is usedSource
metG from E.coli| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter lac operator rbs MetRS(truncated) T7 TE | 2,18 21,44 75,81 89,1219 1389,1436 | promoter feature/promoter feature/operator operator feature/rbs ribosome_entry_site feature/cds CDS stem_loop feature/stem_loop |