Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: You Wang
Date created: 2011-09-28 11:00:00
Date modified: 2015-05-08 01:12:43
metY-CGA
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K567016_sequence (Version 1) |
Description
This biobrick is constructed by mutating the anticodon of tRNA（met） to TCG (base pairing codon CGA). This tRNA can transfer fMet to CGA when it is used as the start codon. Kana gene with start codon substituted for CGA is used to testify the function of metY-CGA. When this biobrick and metGM(BBa_K567014) or metG(BBa_K567015) are co-transformed into the cell, the cells can survive on the LBKana plate.Notes
tRNA(met) has been involved in many operons, we chose metYSource
tRNA（met）from metY in E.coli K12| Sequence Annotation | Location | Component / Role(s) |
| tRNA-Met CAT->TCG -10 -35 PmetY TE TE polya polya | 273,349 307,309 175,181 151,156 151,186 475,490 519,531 491,496 532,538 | CDS feature/cds sequence_alteration feature/mutation promoter feature/promoter feature/promoter promoter promoter feature/promoter stem_loop feature/stem_loop feature/stem_loop stem_loop feature/polya polyA_site feature/polya polyA_site |