BBa_K571000

BBa_K571000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K571000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Lu-Chu Ke

Date created: 2011-10-01 11:00:00

Date modified: 2015-05-08 01:12:44

acsAB/pSB1C3

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K571000_sequence (Version 1)

Description

The acs operon in Gluconacetobacter hansenii is the mechanism that synthesize cellulose synthase, acsAB is one of the gene sequence. The acsAB will produce protein with 2 subunits; one is catalytic subunit which is able to transform UDP-glucose to UDP, another one would be the regulatory subunit of the cellulose synthase which will bind with cyclic di-GMP and activated.
From the primer we design, the acsAB sequence is amplified from PCR from the genomic DNA of Gluconacetobacter hansenii, XbaI and SpeI is also added at the both end of primer as the cutting site, so that acsAB and pSB1C3 could easily bind to it.

Notes

By comparative, acsAB is long base pairs gene, so we need more primer during PCR. It is also advisable to separate PCR into a few times to get the most satisfied results.

Source

*organism: Gluconacetobacter hansenii ATCC 23769
*GenBank: AB091060.1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
acsAB	1,4653	feature/cds, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• rdf-schema#seeAlso: Q76KJ8
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K571000/1

Member of these Collections

• iGEM Parts Registry