Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lu-Chu Ke
Date created: 2011-10-10 11:00:00
Date modified: 2015-05-08 01:12:44
R0011+acsCD+CMCax+Ccp/pSB1C3
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K571010_sequence (Version 1) |
Description
The biobrick is composite element, it is the combination of BBa_K571006 and BBa_K571002. The promoter binding to the upstream of acsCD, CMCax, and Ccp gene, with IPTG as the inducer, activates the genes to synthesize proteins.The sequence of promoter, acsCD, CMCax, and Ccp is amplified by PCR, followed by enzyme digestion and ligation to bind the 4 sequences with the backbone-pSB1C3.
Notes
It is a very long sequence, therefore below is a few measures to take note during cloning.*The concentration agarose gel during gel electrophoresis should be about 0.8-1%
*The enzyme digestion reaction time is advised to be elongated so that digestion could be more perfect.
*The respective restriction enzyme at the both end of primer should not cut the sequence of another gene.
Source
*promoter: iGEM registry of standard biological part*acsCD: GenBank AB091060.1
*CMCax: GenBank EFG85213.1
*Ccp: GenBank AAA16970.1
| Access | Instance | Definition |
| public public | BBa_K571006 BBa_K571002 | BBa_K571006 BBa_K571002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K571006 BBa_K571002 | 1,4449 4456,6547 | BBa_K571006 BBa_K571002 |