BBa_K572005

BBa_K572005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K572005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Avaneesh T
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:44

Proteorhodopsin



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K572005_sequence (Version 1)

Description

Proteorhodopsin, a photon dependent proton pump, is a photoactive retinylidene protein. A transmembrane protein homologous to bacteriorhodopsin in Archaea creates a proton gradient across the cell membrane in many marine organisms. It has been demonstrated that photophosphorylation occurs with the help of transmembrane ATP Synthase on expression of Proteorhodopsin gene in Escherichia coli*

*Martinez A, Bradley AS, Waldbauer JR, Summons RE, DeLong EF (2007). "Proteorhodopsin photosystem gene expression enables photophosphorylation in a heterologous host". PNAS 104 (13): 5590???5595. Bibcode 2007PNAS..104.5590M. doi:10.1073/pnas.0611470104. PMC 1838496. PMID 17372221.

Notes

The gene had E,S and P sites. Thus, we used site directed mutagenesis to optimize the codon and to remove the E,S and P sites so that it can be ligated with the iGEM plasmid backbone.

Source

Source Organism : Marine gamma proteobacterium EBAC31A08
We would like to thank Kwang-Hwan "Kevin" Jung, Ph.D., Associate Professor, Department of Life Science and Institute of Biological Interfaces, Sogang University, Korea for sending us the plasmid (pKJ900) with proteorhodopsin gene.

Sequence Annotation Location Component / Role(s)
Start
Stop
1,3
755,758
sequence_feature feature/misc
sequence_feature feature/misc
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K572005/1