BBa_K572100

BBa_K572100 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K572100

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Chaitra Prabhakara

Date created: 2011-10-04 11:00:00

Date modified: 2015-05-08 01:12:44

Light based screening plasmid backbone - pSB1Pc

Details

• Types: DnaRegion
• Roles: Plasmid_Backbone; plasmid_vector
• Sequences: BBa_K572100_sequence (Version 1)

Description

Screening techniques available presently are all negatively related. The disadvantages of antibiotics are:

1. Principle of Negative Selection
2. Transformants carry a small but unnecessary risk of horizontal gene transfer to environmental microbes
3. Metabolic burden on the cells that significantly reduces the production of the desired protein

Therefore, a novel selectable marker is needed which confers metabolic ???advantage??? to the transformants.

This plasmid carries the gene proteorhodopsin.

Notes

The reading frame had to be maintained.

Source

Source : pSB1C3 is modified. Proteorhodopsin replaces the antibiotic gene.

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K572100/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors