BBa_K576003

BBa_K576003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K576003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: George Zarubin
Date created: 2011-06-19 11:00:00
Date modified: 2015-05-08 01:12:45

Left Part of Self-Excising Ribozyme



    • Details

    Types
    DnaRegion

    Roles
    mature_transcript_region

    RNA

    Sequences BBa_K576003_sequence (Version 1)

    Description

    Modified 3' "half" of intron found in ORF #142 in Twort phage of Staphylococcus aureus in RFC53 MetaPart format. When complemented with 5' "half" (BBa_K576004) in complience with BBFRFC53 assembly standard, the RNA-level excision should leave GGU linker between the whatever comes before K576003 and after K576004. K576003 and K576004 and everything in between them would be removed and possibly circularized.

    Notes

    The P1 helix of the intron has been modified to accommodate the MetaPrefix. The part is in RFC53 L-MetaPart format, having GGT and ATG overgangs.

    Sequence Annotation Location Component / Role(s)
    MetaPrefix
    IN1
    MetaSuffix
    		1,10
    11,119
    120,129
    		feature/dna sequence_feature
    feature/stem_loop stem_loop
    sequence_feature feature/dna

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#topLevel
    BBa_K576003/1
     

    Attachments

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