Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alberto Purwada
Date created: 2011-09-10 11:00:00
Date modified: 2015-05-08 01:12:46
pC+AraC+pBad+GFPc
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K577881_sequence (Version 1) |
Description
This reporter part is a combination of a constitutive promoter, AraC gene, a pBad promoter (inducible with arabinose), and a GFP gene. The whole part is flanked by the regular E, X and S, P restriction sites, so it can be approached with the usual restriction digest method. The purpose of this part is to provide the user with a simple, but complete, inducible genetic device. Adding arabinose with any concentration higher than 0.01 mM seems to cause a significant expression of the GFP gene. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. This part can be inserted in a cell line that does not contain any AraC in its genome, which is important if the user is not using Top 10 cells.Notes
There is no specific design consideration.Source
This part is obtained from ligating several biobricks.| Access | Instance | Definition |
| public public public | BBa_I13458 BBa_I13453 BBa_E0240 | BBa_I13458 BBa_I13453 GFP report |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13458 BBa_I13453 BBa_E0240 | 1,1114 1123,1252 1261,2136 | BBa_I13458 BBa_I13453 GFP report |