Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alberto Purwada
Date created: 2011-09-10 11:00:00
Date modified: 2015-05-08 01:12:46
pC+AraC+pBad+RFPc
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K577882_sequence (Version 1) |
Description
This reporter part is a combination of a constitutive promoter, AraC gene, a pBad promoter (inducible with arabinose), and a RFP gene. The purpose of this part is to provide the user with a simple, but complete, inducible RFP genetic device. Adding arabinose with any concentration higher than 0.01 mM seems to cause a significant expression of the RFP gene. This is useful if the user plans to build a larger plasmid construct in a short time or characterize a specific part. This part can be inserted in a cell line that does not contain any AraC in its genome, which is important if the user is not using Top 10 cells.Notes
The RBS, RFP, and Terminator were taken from BBa_J61002 using SpeI and PstI restriction enzymes.Source
The part is obtained from ligating several biobricks.| Access | Instance | Definition |
| public public public public public | BBa_I13458 BBa_I13453 BBa_B0034 BBa_E1010 BBa_B0015 | BBa_I13458 BBa_I13453 BBa_B0034 mRFP1 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I13458 BBa_I13453 BBa_B0034 BBa_E1010 BBa_B0015 | 1,1114 1123,1252 1261,1272 1279,1984 1993,2121 | BBa_I13458 BBa_I13453 BBa_B0034 mRFP1 BBa_B0015 |