Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kylie Standage-Beier
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:46
T7LRSR (Listeria innocua CLIP11262)
| Types | DnaRegion |
| Roles | sequence_feature Other |
| Sequences | BBa_K578002_sequence (Version 1) |
Description
This brick, T7LRSR, is the starting point for a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) array based around Listeria Innocua CLIP11262. The sequence of the part is composed of a T7 promoter, Lac Operator, Leader Sequence (from Listeria Innocua CLIP11262), Palindromic Repeat, Spacer (Targeted to sequence with correct protospacer and 'NGG; Protospacer-adjacent motif), followed by a palindromic repeat. After the aforementioned sequences there is a pseudospacer with the standard BioBrick suffix (with SpeI and PstI Sites). The suffix allows for parts (eg.Repeat.Spacer Repeat, RSR, formated BioBricks) to be added onto the distal end of the CRISPR array.Notes
This part is intended to be the starting point of a constructible CRISPR array, thus no scars can be introduced in between the Leader Sequence and and initial repeat. Also when constructing an array close to a 100% sequence similarity between the spacer and protospacer is required for CRISPR effector function.Source
This part is based off of the genomic sequence for Listeria Innocua CLIP11262 and was synthesized by Integrate DNA Technologies| Sequence Annotation | Location | Component / Role(s) |
| T7 Promoter/LacO Leader Sequence Repeat Spacer (anti-GFP) Repeat | 1,42 43,170 171,206 207,239 240,275 | promoter feature/promoter feature/conserved conserved_region stem_loop feature/stem_loop sequence_feature feature/misc feature/stem_loop stem_loop |