BBa_K578002

BBa_K578002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K578002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kylie Standage-Beier
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:46

T7LRSR (Listeria innocua CLIP11262)



Types
DnaRegion

Roles
sequence_feature

Other

Sequences BBa_K578002_sequence (Version 1)

Description

This brick, T7LRSR, is the starting point for a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) array based around Listeria Innocua CLIP11262. The sequence of the part is composed of a T7 promoter, Lac Operator, Leader Sequence (from Listeria Innocua CLIP11262), Palindromic Repeat, Spacer (Targeted to sequence with correct protospacer and 'NGG; Protospacer-adjacent motif), followed by a palindromic repeat. After the aforementioned sequences there is a pseudospacer with the standard BioBrick suffix (with SpeI and PstI Sites). The suffix allows for parts (eg.Repeat.Spacer Repeat, RSR, formated BioBricks) to be added onto the distal end of the CRISPR array.

Notes

This part is intended to be the starting point of a constructible CRISPR array, thus no scars can be introduced in between the Leader Sequence and and initial repeat. Also when constructing an array close to a 100% sequence similarity between the spacer and protospacer is required for CRISPR effector function.

Source

This part is based off of the genomic sequence for Listeria Innocua CLIP11262 and was synthesized by Integrate DNA Technologies

Sequence Annotation Location Component / Role(s)
T7 Promoter/LacO
Leader Sequence
Repeat
Spacer (anti-GFP)
Repeat
1,42
43,170
171,206
207,239
240,275
promoter feature/promoter
feature/conserved conserved_region
stem_loop feature/stem_loop
sequence_feature feature/misc
feature/stem_loop stem_loop
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K578002/1