Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reo Yoshimura,Toshihiro Matsunami,Takahiro Nakagawa,Jyunpei Yokoigawa
Date created: 2011-10-04 11:00:00
Date modified: 2015-05-08 01:12:46
no ATG GFP
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K579000_sequence (Version 1) |
Description
This plasmid vector was designed to fuse GFP to C-terminal regions of any proteins.(We use BBa_E0240.)In our projects, we use this plasmid to fuse GFP to dMLF, DIAP2, API2-MALT1 and others to monitor expression of these proteins in E. coli and Drosophila.
Notes
Primer sequenceF:TTGAATTCTTTTCTAGATTTCGTAAAGGAGAAGAA
R:AAACTGCAGAAAACTAGTTTTTTATTATTTGTATAG
Source
820base sequenceAAACCCATAATATCATGACATACCCTATAAAAATAGGCGTATCACGAGGCAGAATTCAGATAAAAAAAATCCCTAGCTTTCGCTAAGGATGATTCTGGAATTCTTTCTAGATTCGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCCAATTCTTGTTGAATTAGATGGTGATGTTAATGGGCACAAATTTTCTGTCAGTGGAGAGGGTGAAGGTGATGCAACATACGGAAAACTTACCCTTAAATTTATTTGCACTACTGGAAAACTACCTGTTCCATGGCCAACACTTGTCACTACTTTCGGTTATGGTGTTCAATGCTTTGCGAGATACCCAGATCATATGAAACAGCATGACTTTTTCAAGAGTGCCATGCCCGAAGGTTATGTACAGGAAAGAACTATATTTTTCAAAGATGACGGGAACTACAAGACACGTGCTGAAGTCAAGTTTGAAGGTGATACCCTTGTTAATAGAATCGAGTTAAAAGGTATTGATTTTAAAGAAGATGGAAACATTCTTGGACACAAATTGGAATACAACTATAACTCACACAATGTATACATCATGGCAGACAAACAAAAGAATGGAATCAAAGTTAACTTCAAAATTAGACACAACATTGAAGATGGAAGCGTTCAACTAGCAGACCATTATCAACAAAATACTCCAATTGGCGATGGCCCTGTCCTTTTACCAGACAACCATTACCTGTCCACACAATCTGCCCTTTCGAAAGATCCCAACGAAAAGAGAGACCACATGGTCCTTCTTGAGTTTGTAACAGCTGCTGGGATTACACATGGCATGGATTTTAATCT
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K579000 no ATG GFP | 1,717 1,717 | engineered_region feature/BioBrick feature/protein CDS |