BBa_K587012

BBa_K587012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K587012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: David Ignacio Maycotte Cervantes

Date created: 2011-10-08 11:00:00

Date modified: 2015-05-08 01:12:47

iTast -> Antikey sequence for high concentration mechanism

Details

• Types: DnaRegion
• Roles: RNA; mature_transcript_region
• Sequences: BBa_K587012_sequence (Version 1)

Description

This sequence forms part of the group of pieces directed to the expression of a concentration mechanism developed by our team ITESM_MEXICO 2011.
The inactivation of gene expression is achieved by transcribing a non-coding RNA (itastRNA) produced by a third promoter (following activation of pBADwk in the presence of arabinose). This mRNA targets the tastRNA specifically. The itastRNA is designed to lock again the complex crstRNA-RBS due to hybridization with tastRNA; inhibiting the expression of the reporter protein at low concentration. It allows only the expression of the high concentration mechanism.

Notes

This is an original design with the objective of enhancing the mechanism designed by British Columbia in 2009.

Source

Isaacs, FJ, Dwyer, DJ, Ding, C, Pervouchine, DD, Cantor, CR, and Collins, JJ. Engineered riboregulators enable post-transcriptional control of gene expression. 2004. Nature Biotechnology 22(7):841-847.

Other Properties

• igem#experience: Issues
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K587012/1

Member of these Collections

• iGEM Parts Registry