KumaMax

BBa_K590087 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K590087

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sydney Gordon, Daniel Hadidi, Elizabeth Stanley, Sarah Wolf, Angus Toland, Sean Wu

Date created: 2011-09-21 11:00:00

Date modified: 2015-05-08 01:12:48

KumaMax: Kumamolisin-As_N291D, G319S, D358G, D368H

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K590087_sequence (Version 1)

Description

A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity with the PQLP peptide, an antigenic epitope in gliadin. This mutant is the best combinatorial mutant found and has point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.

Notes

Aimed to increase binding affinity to the immunogenic PQLP peptide found in gluten

Source

Engineered but the original protein is form Alicyclobacillus sendaiensis

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K590087/1

Member of these Collections

• iGEM Parts Registry