KumaMax

BBa_K590087 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K590087
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sydney Gordon, Daniel Hadidi, Elizabeth Stanley, Sarah Wolf, Angus Toland, Sean Wu
Date created: 2011-09-21 11:00:00
Date modified: 2015-05-08 01:12:48

KumaMax: Kumamolisin-As_N291D, G319S, D358G, D368H



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K590087_sequence (Version 1)

    Description

    A mutated Kumamolisin-As enzyme aimed to combat gluten intolerance by increased activity with the PQLP peptide, an antigenic epitope in gliadin. This mutant is the best combinatorial mutant found and has point mutations at residues 291, 319, 358, and 368 from Asparagine to Aspartate, Glycine to Serine, Aspartate to Glycine, and Aspartate to Histidine, respectively.

    Notes

    Aimed to increase binding affinity to the immunogenic PQLP peptide found in gluten

    Source

    Engineered but the original protein is form Alicyclobacillus sendaiensis

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    BBa_K590087/1
     

    Attachments

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