Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Erik Lundin
Date created: 2011-09-10 11:00:00
Date modified: 2015-05-08 01:12:48
T5-lac Promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K592008_sequence (Version 1) |
Description
T5-lac promoter is a hybrid promoter made from the phage T5 early promoter and lac-operon. It contains three LacI binding sites and remains repressed in LacIq strains where LacI is expressed in high levels. It is inducible by IPTG, and recognizable by E.coli RNA polymerase. Compared to the T7 promoters, T5-lac promoter doesn't require the co-expression of phage polymerase.Notes
The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.Source
Phage T5| Sequence Annotation | Location | Component / Role(s) |
| T5-lac promoter lacO1 site lacO1 site lacOi site -35 signal -10 signal | 1,126 1,26 69,86 94,123 63,68 87,93 | feature/promoter promoter non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/promoter promoter feature/promoter promoter |