leuB

BBa_K594002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K594002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yu Xiaoyan

Date created: 2011-09-29 11:00:00

Date modified: 2015-05-08 01:12:49

subunit of a 3-isopropylmalate dehydrogenase from E.coli BL21

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K594002_sequence (Version 1)

Description

LeuB is essential to leucine biosynthesis, so it is a necessary gene for E.coli's well growth.
The leu operon is responsible for the leucine biosynthesis, including leuA, leuB, leuC, leuD and leuO.
Regulation Summary Diagram:



Summary:
3-isopropylmalate dehydrogenase (LeuB) carries out the third step in leucine biosynthesis, catalyzing the conversion of 3-isopropylmalate to 2-isopropyl-3-oxosuccinate.

3-isopropylmalate dehydrogenase oxidizes 3-isopropylmalate with NAD+ to generate 2-isopropyl-3-oxosuccinate and NADH. The kMs listed below were determined at 40?? C, as was the kcat value of 69/s.

A crystal structure of 3-isopropylmalate dehydrogenase to 2.1 ?? resolution reveals that it is a dimer.

Notes

Besides, when we were looking for leuB gene, we also try to find its RBS, but unluckily, we didn't find its RBS on NCBI, its location is closely linked to leuA's location.

Source

This leuB is from the leu operon in BL 21(E.coli),we get BL21 from Takara and found its sequence in NCBI, then cloned it with a Pfu PCR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
leuB	1,1092	feature/cds, CDS

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• rdf-schema#seeAlso: P30125
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K594002/1

Member of these Collections

• iGEM Parts Registry