rhiI

BBa_K594005 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K594005
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yu Xiaoyan
Date created: 2011-09-30 11:00:00
Date modified: 2015-05-08 01:12:49

a luxI homologous AHL synthetase from Rhzobium leguminosarum bv. vicae IMA57



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K594005_sequence (Version 1)

Description

R. leguminosarum bv. viciae is a best characterized organism in the nitrogen-fixing rhizobia. Several quorum-sensing systems (rai, rhi, cin,and tra) have been identified and are intertwined in a complex regulatory network. The rhi system is composed of rhiR (a luxR homolog),rhiI (a luxI homolog), and the rhiABC operon, all of which are located on the symbiotic plasmid pRL1JI (29, 139). It was demonstrated that rhiABC was controlled by RhiR and that flavonoids repressed the expression of both rhiR and rhiABC.

Notes

Initial experiments showed 3-OH-C14:1-HSL to be an inducer of the rhi genes, but later data showing that rhiI and rhiA could be induced by short-chain AHLs (C6-HSL, C8-HSL, and 3-oxo-C8-HSL) but not 3-OH-C14:1-HSL suggested that the induction was indirect.

Source

it is from the Rhzobium leguminosarum bv. vicae IMA57 we purchased from the CGMCC(China General Microbiological Culture Collection Center).

Sequence Annotation Location Component / Role(s)
rhiI
1,552
CDS feature/cds
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K594005/1