Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yu Xiaoyan
Date created: 2011-09-30 11:00:00
Date modified: 2015-05-08 01:12:49
A device that can accepts the 3--OH-C14:1-HSL and then produces 3-O-C6-HSL and GFP reporter.
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K594014_sequence (Version 1) |
Description
This composite is a device of function. it is composed of ptetR(R0040), RBS(B0034), cinR(C0077), double terminator(B0015), pcin(R0078), RBS(B0030), luxI(C0061), RBS(B0032), GFP(E0030), double terminator(B0015).When IPTG is added, the plac is activated, producing luxR, and then the plux will be activated when 3-O-C6-HSL is added. The 3-O-C6-HSL units with luxR and form a complex that can bind to the lux cassette in plux so that the transcription of lasI, leuB and ECFP happen, producing 3-O-C12-HSL,leuB and the ECFP reporter.
Notes
all the parts are from the iGEM, including K204032, C0261, E0240Source
When ligate the RBS with others, 3A assembly is not as efficient as standard assembly.| Access | Instance | Definition |
| public public public | BBa_K204032 BBa_C0261 BBa_E0240 | BBa_K204032 BBa_C0261 GFP report |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K204032 BBa_C0261 BBa_E0240 | 1,1237 1246,1906 1915,2790 | BBa_K204032 BBa_C0261 GFP report |