BBa_K594015

BBa_K594015 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K594015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Yu Xiaoyan

Date created: 2011-10-04 11:00:00

Date modified: 2015-05-08 01:12:49

A device that can accepts the 3--O-C6-HSL and then produces lasI and EYFP

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K594015_sequence (Version 1)

Description

This composite is a device of function. it is composed of plac(R0011), RBS(B0034), luxR(C0062), double terminator(B0015), plux(R0062), RBS(B0030), lasI(C0078), RBS(B0034), ECFP(E0030), double terminator(B0015).

When IPTG is added, the plac is activated, producing luxR, and then the plux will be activated when 3-O-C6-HSL is added. The 3-O-C6-HSL units with luxR and form a complex that can bind to the lux cassette in plux so that the transcription of lasI and EYFP happen, producing 3-O-C12-HSL and the EYFP reporter.

Notes

NO.

Source

All the parts are from the iGEM.

Components

Access	Instance	Definition
public	BBa_F2622	BBa_F2622
public	BBa_K081009	BBa_K081009
public	BBa_B0034	BBa_B0034
public	BBa_E0030	eyfp
public	BBa_B0015	BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_F2622	1,1062	BBa_F2622
BBa_K081009	1071,1758	BBa_K081009
BBa_B0034	1767,1778	BBa_B0034
BBa_E0030	1785,2507	eyfp
BBa_B0015	2516,2644	BBa_B0015

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K594015/1

Member of these Collections

• iGEM Parts Registry