BBa_K597100

BBa_K597100 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_K597100

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Claire Paduano

Date created: 2011-09-24 11:00:00

Date modified: 2015-05-08 01:12:49

avi-RFP [AviTagged red fluorescent protein]

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K597100_sequence (Version 1)

Description

Avi-RFP codes for RFP with an appended biotinylation marker. When attached to a promoter, it can be used as a red fluorescent reporter which will be biotinylated by E coii. if cultured with biotin. This reporter can then be used in biotin-streptavidin binding applications.

Notes

Avitag sequence was taken from Cull and Schat, 2000.

Source

Avidity, L.L.C., Eleanor Roosevelt Institute, Denver, Colorado 80206, USA.
Ref: Cull, M.G. and Schat, P.J. (2000) "Biotinylation of proteins in vivo and in vitro using small peptide tags."
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Methods of Enzymology Vol 326, 2000, pages 430???440

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
RFP	1,675	CDS, feature/protein
Avitag	688,732	feature/tag, tag
RFP	733,735	CDS, feature/protein
start codon	1,3	feature/start, start_codon

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K597100/1

Member of these Collections

• iGEM Parts Registry