Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: James Mathew
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:50
CCaS and CCaR Light Sensor System
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K597105_sequence (Version 1) |
Description
CCaS and CCaR are integral proteins involved in the green light induced gene expression signaling pathway as described in Tabor et. al. CCaS is a surface protein which, in the presence of 532nm light, becomes photoactivated and begins autophosphorylation when in conjugation with a phytopsycobilin. The phosphate group is then transferred to CCaR, which becomes activated and can bind to Pcpcg2, a light-sensitive promoter, initiating downstream gene expression. CCaS and CCaR are thus a component of a light-induced gene expression system.Cornell's intention with CCaS and CCaR is to ligate this composite with the Ho1, pcyA, and Pcpcg2 genes into a single vector that will function as a light-induced gene expression system. These genes, in addition to CCaS and CCaR, are required for light-sensitive gene expression, as they code for the biosynthesis of the phytopsycobilin.
Notes
CCaS and CCaR are separated by a stop box to prevent transcription run-through. Additionally, there is an RBS after the stop box, to insure the transcription of CCaR. Finally, CCaR is also flanked by a stop box on its C-terminus.Source
Sequences provided by Jeff Tabor (Rice University, Department of BioEngineering)DNA Synthesized through GeneArt, a subsidiary of Invitrogen Life Technologies
Ref: Tabor, J (2011) "Multichromatic control of gene expression in escherichia coli"
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Journal of Molecular Biology Vol. 405, 2, 2011, p. 315
| Access | Instance | Definition |
| public public public | BBa_K592001 BBa_B0030 BBa_K592002 | BBa_K592001 BBa_B0030 BBa_K592002 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K592001 BBa_B0030 BBa_K592002 | 1,2265 2274,2288 2295,3002 | BBa_K592001 BBa_B0030 BBa_K592002 |