BBa_K598024

BBa_K598024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K598024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yan GONG, Xiaowei YAN
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:50

Bistable Switch with TPP Down-regulated Hammerhead Ribozyme 2.5 Regulating CI434 Expression



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K598024_sequence (Version 1)

Description

The down-regulating TPP ribozyme TPP-2.5 affects the green-red ratio of bistable switch by regulating CI434 expression in this part.

Notes

We utilize down-regulating TPP ribozyme TPP-2.5 affecting the green-red ratio of bistable switch in order to test our model.

Source

We construct this part by Gibson Assembly with the bistable switch part from Peking 2007 iGEM team and the part BBa_K598003

Sequence Annotation Location Component / Role(s)
mRFP
RBS
lamda CI repressor
RBS
PRM (CI434 regulated promoter)
PR (CI regulated promoter)
TPP hammerhead ribozyme 2.5 with native RBS
native RBS of TPP 2.5
CI434 repressor
RBS
eGFP
1,681
682,693
708,1421
1422,1433
1440,1491
1492,1540
1545,1694
1682,1694
1695,2330
2341,2352
2353,3072
feature/cds CDS
feature/rbs ribosome_entry_site
CDS feature/cds
ribosome_entry_site feature/rbs
promoter feature/promoter
feature/promoter promoter
sequence_feature feature/dna
feature/rbs ribosome_entry_site
feature/cds CDS
ribosome_entry_site feature/rbs
feature/cds CDS
igem#experience
Works
 
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K598024/1