Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yan GONG, Xiaowei YAN
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:50
Bistable Switch with TPP Down-regulated Hammerhead Ribozyme 2.5 Regulating CI434 Expression
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K598024_sequence (Version 1) |
Description
The down-regulating TPP ribozyme TPP-2.5 affects the green-red ratio of bistable switch by regulating CI434 expression in this part.Notes
We utilize down-regulating TPP ribozyme TPP-2.5 affecting the green-red ratio of bistable switch in order to test our model.Source
We construct this part by Gibson Assembly with the bistable switch part from Peking 2007 iGEM team and the part BBa_K598003| Sequence Annotation | Location | Component / Role(s) |
| mRFP RBS lamda CI repressor RBS PRM (CI434 regulated promoter) PR (CI regulated promoter) TPP hammerhead ribozyme 2.5 with native RBS native RBS of TPP 2.5 CI434 repressor RBS eGFP | 1,681 682,693 708,1421 1422,1433 1440,1491 1492,1540 1545,1694 1682,1694 1695,2330 2341,2352 2353,3072 | feature/cds CDS feature/rbs ribosome_entry_site CDS feature/cds ribosome_entry_site feature/rbs promoter feature/promoter feature/promoter promoter sequence_feature feature/dna feature/rbs ribosome_entry_site feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS |