Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yan GONG, Xiaowei YAN
Date created: 2011-09-27 11:00:00
Date modified: 2015-05-08 01:12:50
Bistable Switch with TPP Hammerhead Ribozyme 2.5 and Theophyline Riboswitch N8-3
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K598025_sequence (Version 1) |
Description
The down-regulating TPP ribozyme TPP 2.5 and Theo N8-3 affect the green-red ratio of bistable switch by regulating CI434 and CI expression respectively in this part.Notes
We utilize down-regulating TPP ribozyme TPP-1.20 and theophylline ribozyme Theo N8-3 affecting the green-red ratio of bistable switch in order to test our model.Source
We construct this part by site mutation with the part BBa_K598024.| Sequence Annotation | Location | Component / Role(s) |
| mRFP RBS lamda CI repressor Theophyline responsive riboswitch N8-3 with native RBS native RBS of THeo N8-3 PRM (CI434 regulated promoter) TPP hammerhead ribozyme 1.20 with native RBS PR (CI regulated promoter) native RBS of TPP 1.20 CI434 repressor RBS eGFP | 1,681 682,693 708,1421 1422,1477 1422,1433 1484,1535 1589,1738 1536,1584 1727,1738 1739,2374 2385,2396 2397,3116 | CDS feature/cds feature/rbs ribosome_entry_site feature/cds CDS sequence_feature feature/dna ribosome_entry_site feature/rbs promoter feature/promoter sequence_feature feature/dna promoter feature/promoter ribosome_entry_site feature/rbs feature/cds CDS feature/rbs ribosome_entry_site CDS feature/cds |