BBa_K606045

BBa_K606045 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K606045
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Cyrille Pauthenier
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:51

T7 RNA polymerase double amber



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Description

    This protein is a T7 RNA polymerase from bacteriophage that have been amber mutated in two distinct tyrosine sites.

    Notes

    The quick change mutagenesis has been done with a single long primer of 94 bp. So the mutagenesis is quite hard to do.

    Source

    The part K145001 has been mutated in two tyrosine site using Site Directed Quick Change mutagenesis.

    igem#experience
    None
     
    igem#sampleStatus
    Discontinued
    igem#status
    Deleted
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K606045/1
     

    Attachments

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