Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ke Liu
Date created: 2011-09-30 11:00:00
Date modified: 2015-05-08 01:12:53
nisI+pH induced promoter rcfB+ repressor LacR of lac operon
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K609000_sequence (Version 1) |
Description
When the external pH value declines to 5.5, rcfB promoter will be highly upregulated. Along with that, the lacR gene, which is downstream of rcfB, will be highly transcribed. The large translation of LacR, the repressor of lac operon in Lactobacillus delbrueckii, will inhibit the use of lactose in the bacteria.And the nisI gene is a Nisin resistance gene which could be used in food.Notes
The part is designed to let Lactobacillus bulgaricus stop producing lactic acid when the external pH declines to 5.5 or lower.Source
The nisI gene comes from the Staphylococcus aureuis. The rcfB promoter is found in Lactococcus lactis IL1403, while the lacR gene is from Lactobacillus. delbrueckii subsp. lactis.| Access | Instance | Definition |
| public public | BBa_K609003 BBa_K609001 | BBa_K609003 BBa_K609001 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K609003 BBa_K609001 | 1,869 878,2017 | BBa_K609003 BBa_K609001 |