BBa_K611008

BBa_K611008 Version 1

Component

This part has been discontinued.

Please consider using BBa_R0040 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K611008
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Csicsery
Date created: 2011-09-17 11:00:00
Date modified: 2015-05-08 01:12:53

R0040+B0034+E0040+B0015+I13453+B0034



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K611008_sequence (Version 1)

Description

This part is used to characterize mutants of BBa_C0040 (TetR). By adding a mutant of BBa_C0040 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added TetR mutant can be varied through different arabinose concentrations as long as AraC is present.

Notes

There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3).

Source

Built from existing parts in the registry though BioBrick assembly.

Sequence Annotation Location Component / Role(s)
BBa_R0040
TetR 1
-35
TetR 2
-10
1,54
1,19
20,25
26,44
43,48
feature/BioBrick engineered_region
non_covalent_binding_site feature/binding
feature/promoter promoter
non_covalent_binding_site feature/binding
feature/promoter promoter
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
Discontinued
igem#status
Deleted
 
isReplacedBy
BBa_R0040/1
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K611008/1