Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Csicsery
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:53
LacI Promoter Variant #1
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K611021_sequence (Version 1) |
Description
Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs.Notes
We chose to mutate this part from a well known promoter so that any changes in behavior would be easy to observe.Source
Mutagenic PCR of BBa_R0010.| Sequence Annotation | Location | Component / Role(s) |
| t->a a->g CAP binding site a->g a->g -35 t->c -10 LacI binding site t->c a->t start end of LacI coding region (inactive) | 37,37 40,40 89,126 103,103 108,108 137,142 151,151 161,166 166,200 176,176 196,196 173,173 1,88 | sequence_alteration feature/mutation feature/mutation sequence_alteration non_covalent_binding_site feature/binding sequence_alteration feature/mutation sequence_alteration feature/mutation promoter feature/promoter feature/mutation sequence_alteration feature/promoter promoter non_covalent_binding_site feature/binding sequence_alteration feature/mutation feature/mutation sequence_alteration start_codon feature/start feature/cds CDS |