BBa_K611022

BBa_K611022 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K611022

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nicholas Csicsery

Date created: 2011-09-18 11:00:00

Date modified: 2015-05-08 01:12:53

LacI Promoter Variant #2

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K611022_sequence (Version 1)

Description

Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs.

Notes

We chose to mutate this part from a well known promoter so that any changes in behavior would be easy to observe.

Source

Mutagenic PCR of BBa_R0010.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
a->t	103,103	feature/mutation, sequence_alteration
a->g	198,198	feature/mutation, sequence_alteration
LacI binding site	166,200	feature/binding, non_covalent_binding_site
end of LacI coding region (inactive)	1,88	feature/cds, CDS
CAP binding site	89,126	non_covalent_binding_site, feature/binding
-35	137,142	promoter, feature/promoter
-10	161,166	promoter, feature/promoter
start	173,173	start_codon, feature/start

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K611022/1

Member of these Collections

• iGEM Parts Registry