BBa_K611023

BBa_K611023 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K611023
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Csicsery
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:53

LacI Promoter Variant #3



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K611023_sequence (Version 1)

Description

Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs.

Notes

We chose to mutate this part from a well known promoter so that any changes in behavior would be easy to observe.

Source

Mutagenic PCR of BBa_R0010.

Sequence Annotation Location Component / Role(s)
t->a
t->a
LacI binding site
end of LacI coding region (inactive)
CAP binding site
-35
-10
start
104,104
148,148
166,200
1,88
89,126
137,142
161,166
173,173
feature/mutation sequence_alteration
sequence_alteration feature/mutation
non_covalent_binding_site feature/binding
CDS feature/cds
feature/binding non_covalent_binding_site
promoter feature/promoter
promoter feature/promoter
start_codon feature/start
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K611023/1