Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Csicsery
Date created: 2011-09-18 11:00:00
Date modified: 2015-05-08 01:12:53
LacI Promoter Variant #5
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K611025_sequence (Version 1) |
Description
Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs.Notes
We chose to mutate this part from a well known promoter so that any changes in behavior would be easy to observe.Source
Mutagenic PCR of BBa_R0010.| Sequence Annotation | Location | Component / Role(s) |
| t->c a->c t->c LacI binding site end of LacI coding region (inactive) CAP binding site -35 -10 start | 30,30 117,117 123,123 166,200 1,88 89,126 137,142 161,166 173,173 | feature/mutation sequence_alteration sequence_alteration feature/mutation feature/mutation sequence_alteration non_covalent_binding_site feature/binding feature/cds CDS non_covalent_binding_site feature/binding promoter feature/promoter feature/promoter promoter feature/start start_codon |