BBa_K611033

BBa_K611033 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K611033
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nicholas Csicsery
Date created: 2011-09-26 11:00:00
Date modified: 2015-05-08 01:12:53

LacI Promoter Variant #3 in Characterization Construct



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K611033_sequence (Version 1)

Description

This part contains a variant of the wild type LacI repressible promoter, and is followed by the LacI characterization construct, K611013. This allows for testing of this particular mutant promoter at various concentrations of repressor. The mutated LacI promoter in this plasmid expresses GFP and is followed by an arabinose inducible promoter, pBad, which expresses the LacI repressor. Thus, a user can increase the concentration of repressor by inducing pBad with arabinose. Similarly, the repressor may be unrepressed with the addition of IPTG. This plasmid is very useful for the further characterization of K611023.

Notes

This part does not have a termination sequence at the end. It is recommended that for optimal results, this part be used in a backbone that includes a terminator (such as pSB1C3).

Source

Mutagenic PCR of the LacI repressible promoter, R0010, and standard BioBrick assembly of parts from the registry.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K611033/1